Hotspot

psbA

LSC · 262-1323 · 1062 bp

Back to Heliconia

Pi 0.00226

MarkerSeek score 30.40

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 5
pi: 0.00226
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 30.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	ATGTATGTTGTGCGCAAAAA	CATAGGGAAAGTCGTGTGCA	35.0	50.0	55.1	57.9	1562-1567 bp	1.0	56.9
psbA_p2	ATGTATGTTGTGCGCAAAAA	ACATAGGGAAAGTCGTGTGC	35.0	50.0	55.1	57.9	1563-1568 bp	1.0	56.9
psbA_p3	ATGTATGTTGTGCGCAAAAA	AATACGTGTGCTTGGGAGTC	35.0	50.0	55.1	57.9	1238-1243 bp	1.0	56.8
psbA_p4	CAAACAAAGTAGCAATACCCCA	CATAGGGAAAGTCGTGTGCA	40.9	50.0	56.9	57.9	1529-1534 bp	1.0	55.2
psbA_p5	CAAACAAAGTAGCAATACCCCA	AATACGTGTGCTTGGGAGTC	40.9	50.0	56.9	57.9	1205-1210 bp	1.0	55.1