Hotspot

rps15-ycf1

SSC · 130474-130872 · 399 bp

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Pi 0.0291

MarkerSeek score 61.80

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 399
variable_sites: 20
pi: 0.0291
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	GGCTCAGCTCCAGCATAATT	GGACGACCCCTTGAATGAAA	50.0	50.0	58.0	57.8	1788-1795 bp	1.0	80.1
rps15-ycf1_p2	AACACCATGCATTGATGGGT	GGACGACCCCTTGAATGAAA	45.0	50.0	58.1	57.8	1535-1542 bp	1.0	80.0
rps15-ycf1_p3	GAAGCTATACGGCTCAGCTC	GGACGACCCCTTGAATGAAA	55.0	50.0	57.9	57.8	1798-1805 bp	1.0	79.8
rps15-ycf1_p4	GAGAAGCTATACGGCTCAGC	GGACGACCCCTTGAATGAAA	55.0	50.0	57.9	57.8	1800-1807 bp	1.0	79.8
rps15-ycf1_p5	ACAGAACCCCCGTTTTCTTC	GGACGACCCCTTGAATGAAA	50.0	50.0	58.0	57.8	1157-1164 bp	0.6	64.2