Hotspot

psaC-ndhE

SSC · 123797-124720 · 924 bp

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Pi 0.0171

MarkerSeek score 63.70

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 924
variable_sites: 33
pi: 0.0171
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	CAATACATCTGTGGGGCAGG	TCGAAACAGAAAATCAACTCGT	55.0	36.4	58.3	56.6	957-1070 bp	1.0	56.9
psaC-ndhE_p2	CCAATACATCTGTGGGGCAG	TCGAAACAGAAAATCAACTCGT	55.0	36.4	58.3	56.6	958-1071 bp	1.0	56.9
psaC-ndhE_p3	CAGGCTCGTACACATTGAGT	TCGAAACAGAAAATCAACTCGT	50.0	36.4	57.6	56.6	941-1054 bp	1.0	56.5
psaC-ndhE_p4	TTCCAATACATCTGTGGGGC	TCGAAACAGAAAATCAACTCGT	50.0	36.4	57.6	56.6	960-1073 bp	1.0	56.0
psaC-ndhE_p5	TCCAATACATCTGTGGGGCA	TCGAAACAGAAAATCAACTCGT	50.0	36.4	58.7	56.6	959-1072 bp	1.0	53.8