Hotspot

ccsA-ndhD

SSC · 121639-121921 · 283 bp

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Pi 0.0249

MarkerSeek score 61.80

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 283
variable_sites: 17
pi: 0.0249
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	ATTCAGCACTTGTGGCTTCT	CTCGCTATCAGTTGACAGGG	45.0	55.0	57.7	57.8	452-454 bp	1.0	79.4
ccsA-ndhD_p2	ATTCAGCACTTGTGGCTTCT	TCGCTATCAGTTGACAGGGT	45.0	50.0	57.7	58.4	451-453 bp	1.0	77.6
ccsA-ndhD_p3	ATTCAGCACTTGTGGCTTCT	TCAGTTGACAGGGTACAAGC	45.0	50.0	57.7	57.5	445-447 bp	1.0	76.8
ccsA-ndhD_p4	ATTCAGCACTTGTGGCTTCT	CAGTTGACAGGGTACAAGCT	45.0	50.0	57.7	57.4	444-446 bp	1.0	76.7
ccsA-ndhD_p5	TTCAGCACTTGTGGCTTCTA	CTCGCTATCAGTTGACAGGG	45.0	55.0	57.1	57.8	451-453 bp	1.0	74.2