Hotspot

trnH-GUG-rpl2

IRb · 90014-90056 · 43 bp

Back to Heliconia

Pi 0.0744

MarkerSeek score 68.70

Primer available yes

Label trnH-GUG-rpl2

Feature Metadata

label_name: trnH-GUG-rpl2
feature_type: igs
region: IRb
length_bp: 43
variable_sites: 5
pi: 0.0744
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-rpl2_p1	TGGATCAAGGCAGTGGATTG	GTTATCCTGCGCTTGGAAGA	50.0	50.0	57.9	58.0	171-179 bp	1.0	84.7
trnH-GUG-rpl2_p2	GGATCAAGGCAGTGGATTGT	GTTATCCTGCGCTTGGAAGA	50.0	50.0	57.9	58.0	170-178 bp	1.0	84.7
trnH-GUG-rpl2_p3	GTGGATCAAGGCAGTGGATT	GTTATCCTGCGCTTGGAAGA	50.0	50.0	57.9	58.0	172-180 bp	1.0	84.6
trnH-GUG-rpl2_p4	CGGATGTAGCCAAGTGGATC	GTTATCCTGCGCTTGGAAGA	55.0	50.0	58.1	58.0	185-193 bp	1.0	84.3
trnH-GUG-rpl2_p5	CGGATGTAGCCAAGTGGATC	CACAACCCCTTGGGGTTATC	55.0	55.0	58.1	58.2	199-207 bp	1.0	82.9