Hotspot

trnT-GGU-psbD

LSC · 35831-36933 · 1103 bp

Back to Heliconia

Pi 0.0102

MarkerSeek score 52.10

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1103
variable_sites: 24
pi: 0.0102
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	AAGGAAAGAGCAATAGGCCG	55.0	50.0	58.1	58.0	1282-1300 bp	1.0	81.1
trnT-GGU-psbD_p2	GTGGTAGAGTAACGCCATGG	CACCGTCACCATCTTCGAAT	55.0	50.0	58.1	58.0	1849-1867 bp	1.0	80.8
trnT-GGU-psbD_p3	GGGGTCGGTCTTGAAACAAT	CACCGTCACCATCTTCGAAT	50.0	50.0	58.1	58.0	2084-2104 bp	1.0	80.8
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	CACCGTCACCATCTTCGAAT	50.0	50.0	58.1	58.0	1852-1870 bp	1.0	80.6
trnT-GGU-psbD_p5	AACGGGCCCTTTTAACTCAG	CACCGTCACCATCTTCGAAT	50.0	50.0	58.1	58.0	1868-1886 bp	1.0	80.6