Hotspot

psbM-trnD-GUC

LSC · 33315-34337 · 1023 bp

Back to Heliconia

Pi 0.00899

MarkerSeek score 64.00

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1023
variable_sites: 21
pi: 0.00899
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	ATGAACAGCGCAGTAGCAAT	CCTCGCTGGGATTGTAGTTC	45.0	55.0	58.3	58.1	1119-1157 bp	1.0	81.8
psbM-trnD-GUC_p2	ATGAACAGCGCAGTAGCAAT	AGTTCAATCGGTCAGAGCAC	45.0	50.0	58.3	57.9	1104-1142 bp	1.0	81.6
psbM-trnD-GUC_p3	ATGAACAGCGCAGTAGCAAT	TACACCTCGCTGGGATTGTA	45.0	50.0	58.3	58.1	1123-1161 bp	1.0	81.1
psbM-trnD-GUC_p4	TGAACAGCGCAGTAGCAATA	CCTCGCTGGGATTGTAGTTC	45.0	55.0	57.6	58.1	1118-1156 bp	1.0	80.8
psbM-trnD-GUC_p5	TGAACAGCGCAGTAGCAATA	AGTTCAATCGGTCAGAGCAC	45.0	50.0	57.6	57.9	1103-1141 bp	1.0	80.7