Hotspot

atpF-atpH

LSC · 15024-15538 · 515 bp

Back to Heliconia

Pi 0.0099

MarkerSeek score 64.50

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 515
variable_sites: 10
pi: 0.0099
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CAGTGTCCCAAGGAAACGAA	AATTTACGGACTGGTCGTGG	50.0	50.0	58.0	57.9	598-617 bp	1.0	82.1
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	AATTTACGGACTGGTCGTGG	50.0	50.0	57.9	57.9	626-645 bp	1.0	81.4
atpF-atpH_p3	CAGTGTCCCAAGGAAACGAA	TCGTGGCATTAGCGCTTTTA	50.0	45.0	58.0	58.3	584-603 bp	1.0	80.8
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	TCGTGGCATTAGCGCTTTTA	50.0	45.0	57.9	58.3	612-631 bp	1.0	80.0
atpF-atpH_p5	CAGTGTCCCAAGGAAACGAA	CGTGGCATTAGCGCTTTTAT	50.0	45.0	58.0	57.2	583-602 bp	1.0	76.9