Hotspot

trnS-GCU-trnG-GCC

LSC · 9268-10926 · 1659 bp

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Pi 0.0139

MarkerSeek score 63.10

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 1659
variable_sites: 47
pi: 0.0139
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	ATTAGCAATCCGCCGCTTTA	AACTGAGAGACCCCTTAACT	45.0	45.0	58.0	55.1	1764-1862 bp	1.0	59.7
trnS-GCU-trnG-GCC_p2	TCAAGTAATCAGAGCGAGCG	AACTGAGAGACCCCTTAACT	50.0	45.0	57.8	55.1	1830-1928 bp	1.0	58.3
trnS-GCU-trnG-GCC_p3	ATTAGCAATCCGCCGCTTTA	AAACTGAGAGACCCCTTAACT	45.0	42.9	58.0	55.9	1765-1863 bp	1.0	58.3
trnS-GCU-trnG-GCC_p4	CGGAGAGAGAGGGATTCGAA	AACTGAGAGACCCCTTAACT	55.0	45.0	58.3	55.1	1812-1910 bp	1.0	57.3
trnS-GCU-trnG-GCC_p5	TCAAGTAATCAGAGCGAGCG	AAACTGAGAGACCCCTTAACT	50.0	42.9	57.8	55.9	1831-1929 bp	1.0	57.0