Hotspot

trnK-UUU-rps16

LSC · 4253-4995 · 743 bp

Back to Heliconia

Pi 0.00951
MarkerSeek score 66.20
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
743
variable_sites
14
pi
0.00951
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT CGCTCAACCAACAGGAACT 50.0 52.6 58.2 58.0 843-863 bp 1.0 73.1
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT CGCTCAACCAACAGGAACT 50.0 52.6 58.2 58.0 842-862 bp 1.0 73.1
trnK-UUU-rps16_p3 TACCGTTGAGTTAGCAACCC CGCTCAACCAACAGGAACT 50.0 52.6 57.5 58.0 829-849 bp 1.0 70.8
trnK-UUU-rps16_p4 AGTCGCACTTAAAAGCCGAG CGCTCAACCAACAGGAACT 50.0 52.6 58.6 58.0 854-874 bp 1.0 69.9
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT CTTGAAAAGGGCGCTCAAC 50.0 52.6 58.2 57.5 853-873 bp 1.0 69.3