Hotspot

ycf3

LSC · 43981-45975 · 1995 bp

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Pi 0

MarkerSeek score 57.80

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 1995
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	ACTGATGGAGCCAACAAACA	GGTATCCTTTTACCACGAGAGG	45.0	50.0	57.6	57.9	2097-2098 bp	1.0	60.7
ycf3_p2	ACTGATGGAGCCAACAAACA	ATCCTTTTACCACGAGAGGT	45.0	45.0	57.6	55.9	2094-2095 bp	1.0	60.0
ycf3_p3	ACTGATGGAGCCAACAAACA	ACCACGAGAGGTAATGAGAAG	45.0	47.6	57.6	56.8	2086-2087 bp	1.0	59.9
ycf3_p4	ACTGATGGAGCCAACAAACA	ACCACGAGAGGTAATGAGAAGA	45.0	45.5	57.6	58.3	2086-2087 bp	1.0	58.7
ycf3_p5	ACTGATGGAGCCAACAAACA	CGTGCTGTAACTGTACAATTCC	45.0	45.5	57.6	57.7	2133-2134 bp	1.0	58.6