Hotspot

rps2

LSC · 15400-16110 · 711 bp

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Pi 0.00141

MarkerSeek score 46.80

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 711
variable_sites: 1
pi: 0.00141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	TGGAGTTCCCGAAGTAATTGA	AAAGAGGAAGTGTGGAGAGA	42.9	45.0	56.7	55.4	769 bp	1.0	40.6
rps2_p2	TGGAGTTCCCGAAGTAATTGA	AAGAGGAAGTGTGGAGAGAA	42.9	45.0	56.7	55.4	768 bp	1.0	40.6
rps2_p3	TGGAGTTCCCGAAGTAATTGA	AAAAGAGGAAGTGTGGAGAGA	42.9	42.9	56.7	56.2	770 bp	1.0	40.1
rps2_p4	TGGAGTTCCCGAAGTAATTGA	AAAGAGGAAGTGTGGAGAGAA	42.9	42.9	56.7	56.2	769 bp	1.0	40.1
rps2_p5	TCCCCAGTGATTTTGATCTCT	AAGAGGAAGTGTGGAGAGAA	42.9	45.0	56.3	55.4	829 bp	1.0	40.1