Hotspot

rps16

LSC · 5210-6319 · 1110 bp

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Pi 0

MarkerSeek score 37.80

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1110
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AGCTAGATCTAAGGGTGACTCC	TGCTGGTTCCAAATTAGAGGA	50.0	42.9	58.2	56.9	1191 bp	1.0	46.0
rps16_p2	GCTAGATCTAAGGGTGACTCC	TGCTGGTTCCAAATTAGAGGA	52.4	42.9	56.7	56.9	1190 bp	1.0	44.9
rps16_p3	AGCTAGATCTAAGGGTGACTCC	TGCTGGTTCCAAATTAGAGGAA	50.0	40.9	58.2	57.6	1191 bp	1.0	43.7
rps16_p4	AGCTAGATCTAAGGGTGACTCC	TCAATTTCATGCTGGTTCCA	50.0	40.0	58.2	55.5	1200 bp	1.0	42.9
rps16_p5	GCTAGATCTAAGGGTGACTCC	TGCTGGTTCCAAATTAGAGGAA	52.4	40.9	56.7	57.6	1190 bp	1.0	42.6