Hotspot

rpoA

LSC · 79213-80199 · 987 bp

Back to Helicia

Pi 0.00811

MarkerSeek score 63.40

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 987
variable_sites: 8
pi: 0.00811
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	TGTGCTGAAGATTCAAAACCC	ACATAATGGGTGTAGACCTCCT	42.9	45.5	57.0	58.0	1195 bp	1.0	48.4
rpoA_p2	TGTGCTGAAGATTCAAAACCC	CACATAATGGGTGTAGACCTCC	42.9	50.0	57.0	57.9	1196 bp	1.0	47.9
rpoA_p3	TGTGCTGAAGATTCAAAACCC	CCACATAATGGGTGTAGACCTC	42.9	50.0	57.0	57.9	1197 bp	1.0	47.9
rpoA_p4	TGTGCTGAAGATTCAAAACCC	CCACATAATGGGTGTAGACCT	42.9	47.6	57.0	56.8	1197 bp	1.0	46.8
rpoA_p5	GTGCTGAAGATTCAAAACCCT	ACATAATGGGTGTAGACCTCCT	42.9	45.5	56.7	58.0	1194 bp	1.0	46.1