Hotspot

clpP

LSC · 71196-73260 · 2065 bp

Back to Helicia

Pi 0.000484

MarkerSeek score 57.90

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2065
variable_sites: 1
pi: 0.000484
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TTCGAAATGGAATCGCGGAT	ACCTTCTTACGTTTCCACATCA	45.0	40.9	58.1	57.7	2146 bp	1.0	60.9
clpP_p2	TTCGAAATGGAATCGCGGAT	ACGTTTCCACATCAAAGTGA	45.0	40.0	58.1	55.5	2138 bp	1.0	59.8
clpP_p3	TTCGAAATGGAATCGCGGAT	ACGTTTCCACATCAAAGTGAAG	45.0	40.9	58.1	57.4	2138 bp	1.0	59.2
clpP_p4	TTCGAAATGGAATCGCGGAT	CGTTTCCACATCAAAGTGAAGT	45.0	40.9	58.1	57.4	2137 bp	1.0	59.2
clpP_p5	TTCGAAATGGAATCGCGGAT	ACGTTTCCACATCAAAGTGAA	45.0	38.1	58.1	56.3	2138 bp	1.0	58.1