Hotspot

cemA

LSC · 62418-63107 · 690 bp

Back to Helicia

Pi 0.00145

MarkerSeek score 59.60

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: LSC
length_bp: 690
variable_sites: 1
pi: 0.00145
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	GAGTCGACAAATTAGGTGGGT	AGATTAATGACGGTGGACCT	47.6	45.0	57.7	55.9	759 bp	1.0	52.9
cemA_p2	TCAGATCGGATAGAGTCGACA	AGATTAATGACGGTGGACCT	47.6	45.0	57.5	55.9	771 bp	1.0	51.8
cemA_p3	AGTCGACAAATTAGGTGGGT	AGATTAATGACGGTGGACCT	45.0	45.0	56.5	55.9	758 bp	1.0	51.2
cemA_p4	GAGTCGACAAATTAGGTGGGT	AGATTAATGACGGTGGACCTT	47.6	42.9	57.7	56.7	759 bp	1.0	51.2
cemA_p5	AGAGTCGACAAATTAGGTGGG	AGATTAATGACGGTGGACCT	47.6	45.0	57.4	55.9	760 bp	1.0	50.7