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rpl16-rps3

LSC · 82983-84105 · 1123 bp

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Pi 0.000891

MarkerSeek score 59.30

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 1123
variable_sites: 1
pi: 0.000891
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	GAAGAGCGTATCTGCCGAAA	CCCTACAAACCATTCGAGCT	50.0	50.0	58.1	57.6	1332-1333 bp	1.0	76.3
rpl16-rps3_p2	AACAAATACGATTGCCCCGA	CCCTACAAACCATTCGAGCT	45.0	50.0	57.9	57.6	1314-1315 bp	1.0	76.0
rpl16-rps3_p3	AGCGTATCTGCCGAAACAAA	CCCTACAAACCATTCGAGCT	45.0	50.0	58.2	57.6	1328-1329 bp	1.0	75.3
rpl16-rps3_p4	GAAGAGCGTATCTGCCGAAA	GGGTTCCCCTACAAACCATT	50.0	50.0	58.1	57.4	1338-1339 bp	1.0	74.6
rpl16-rps3_p5	GAAGAGCGTATCTGCCGAAA	CCCCTACAAACCATTCGAGC	50.0	55.0	58.1	58.6	1333-1334 bp	1.0	74.5