Hotspot

rbcL-accD

LSC · 57496-58234 · 739 bp

Back to Helicia

Pi 0.00677

MarkerSeek score 62.60

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 739
variable_sites: 5
pi: 0.00677
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCTGCTTGTGAGGTATGGAA	CACTTCCGCTGGTATTTCCA	50.0	50.0	57.9	57.9	911 bp	1.0	78.7
rbcL-accD_p2	GCTGCTTGTGAGGTATGGAA	CACCAAGCCTGTCCATTGAT	50.0	50.0	57.9	58.2	887 bp	1.0	78.3
rbcL-accD_p3	GCTGCTTGTGAGGTATGGAA	TCACTTCCGCTGGTATTTCC	50.0	50.0	57.9	57.6	912 bp	1.0	76.4
rbcL-accD_p4	TTCGAATTCCCAGCAATGGA	CACTTCCGCTGGTATTTCCA	45.0	50.0	57.5	57.9	881 bp	1.0	75.5
rbcL-accD_p5	TTCGAATTCCCAGCAATGGA	CACCAAGCCTGTCCATTGAT	45.0	50.0	57.5	58.2	857 bp	1.0	75.2