Hotspot

atpB-rbcL

LSC · 55275-56067 · 793 bp

Back to Helicia

Pi 0.00128

MarkerSeek score 61.20

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 793
variable_sites: 1
pi: 0.00128
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	AAACCCCAGGACCAGAAGTA	AATGCAGCCAAGATATCCGT	50.0	45.0	57.9	57.4	935-949 bp	1.0	75.1
atpB-rbcL_p2	GGACCGATGATTTGAGCGAT	AATGCAGCCAAGATATCCGT	50.0	45.0	58.1	57.4	984-998 bp	1.0	75.0
atpB-rbcL_p3	AACCCCAGGACCAGAAGTAG	AATGCAGCCAAGATATCCGT	55.0	45.0	58.3	57.4	934-948 bp	1.0	73.4
atpB-rbcL_p4	CCCCAGGACCAGAAGTAGTA	AATGCAGCCAAGATATCCGT	55.0	45.0	57.5	57.4	932-946 bp	1.0	71.8
atpB-rbcL_p5	GTACCGGACCGATGATTTGA	AATGCAGCCAAGATATCCGT	50.0	45.0	57.4	57.4	989-1003 bp	1.0	71.3