Hotspot

psaA-ycf3

LSC · 43241-43980 · 740 bp

Back to Helicia

Pi 0

MarkerSeek score 59.50

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 740
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	TTGGTTGAAGATCACGAGGC	50.0	50.0	57.9	58.2	845-851 bp	1.0	78.1
psaA-ycf3_p2	GAGAAATGACCCGGTCTAGC	TTGGTTGAAGATCACGAGGC	55.0	50.0	57.8	58.2	863-869 bp	1.0	77.2
psaA-ycf3_p3	GGCCCTTCGCTATTGTTCTT	TTGGTTGAAGATCACGAGGC	50.0	50.0	58.2	58.2	883-889 bp	1.0	77.0
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	GCTATAGCGCTTACTCCAGG	50.0	55.0	57.9	57.6	886-892 bp	1.0	76.8
psaA-ycf3_p5	GAGAAATGACCCGGTCTAGC	GCTATAGCGCTTACTCCAGG	55.0	55.0	57.8	57.6	904-910 bp	1.0	75.9