Hotspot

tRNA-Ser(GCT)-tRNA-Arg(TCT)

LSC · 8083-9765 · 1683 bp

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Pi 0.0006

MarkerSeek score 59.70

Primer available yes

Label tRNA-Ser(GCT)-tRNA-Arg(TCT)

Feature Metadata

label_name: tRNA-Ser(GCT)-tRNA-Arg(TCT)
feature_type: igs
region: LSC
length_bp: 1683
variable_sites: 1
pi: 0.0006
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Ser(GCT)-tRNA-Arg(TCT)_p1	CAACGGATTAGCAATTCGCC	AGAAGACCTCTGTCCTATCCA	50.0	47.6	57.9	56.9	1748-1765 bp	1.0	63.6
tRNA-Ser(GCT)-tRNA-Arg(TCT)_p2	ACGGAAAGAGAGGGATTCGA	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.2	56.9	1791-1808 bp	1.0	63.2
tRNA-Ser(GCT)-tRNA-Arg(TCT)_p3	CAACGGATTAGCAATTCGCC	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	57.9	57.9	1754-1771 bp	1.0	62.9
tRNA-Ser(GCT)-tRNA-Arg(TCT)_p4	ACGGAAAGAGAGGGATTCGA	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.2	57.9	1797-1814 bp	1.0	62.5
tRNA-Ser(GCT)-tRNA-Arg(TCT)_p5	ATTAGCAATTCGCCGCTTTC	AGAAGACCTCTGTCCTATCCA	45.0	47.6	57.5	56.9	1742-1759 bp	1.0	60.6