Hotspot

trnQ-UUG-rps16

LSC · 76874-77788 · 915 bp

Back to Helichrysum

Pi 0.00656
MarkerSeek score 62.50
Primer available yes
Label trnQ-UUG-rps16

Feature Metadata

label_name
trnQ-UUG-rps16
feature_type
igs
region
LSC
length_bp
915
variable_sites
6
pi
0.00656
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-rps16_p1 GAGGTTCGAATCCTTCCGTC GGGATCGTGTCCTTCAAGTC 55.0 55.0 58.1 58.0 1003-1044 bp 1.0 79.8
trnQ-UUG-rps16_p2 GAGGTTCGAATCCTTCCGTC AAGTCGCACGTTGCTTTCTA 55.0 45.0 58.1 58.1 988-1029 bp 1.0 78.8
trnQ-UUG-rps16_p3 GAGGTTCGAATCCTTCCGTC GGATCGTGTCCTTCAAGTCG 55.0 55.0 58.1 58.4 1002-1043 bp 1.0 76.9
trnQ-UUG-rps16_p4 GAGGTTCGAATCCTTCCGTC CCAAGAGCACCTTCATTCCT 55.0 50.0 58.1 57.5 1052-1093 bp 1.0 75.8
trnQ-UUG-rps16_p5 TTCGGAGGTTCGAATCCTTC GGGATCGTGTCCTTCAAGTC 50.0 55.0 57.3 58.0 1007-1048 bp 1.0 75.0