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psbM-petN

LSC · 73477-73977 · 501 bp

Back to Helichrysum

Pi 0.00815

MarkerSeek score 63.30

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 501
variable_sites: 4
pi: 0.00815
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TGCTACTGCACTGTTCATTT	TAGTAAGTCTCGCTTGGGCT	40.0	50.0	55.6	58.2	663-677 bp	1.0	61.1
psbM-petN_p2	TGCTACTGCACTGTTCATTTT	TAGTAAGTCTCGCTTGGGCT	38.1	50.0	56.4	58.2	663-677 bp	1.0	59.4
psbM-petN_p3	TGCTACTGCACTGTTCATTT	ATAGTAAGTCTCGCTTGGGC	40.0	50.0	55.6	57.1	664-678 bp	1.0	55.1
psbM-petN_p4	TGCTACTGCACTGTTCATTT	CTCGCTTGGGCTGCTTTAAT	40.0	50.0	55.6	58.9	655-669 bp	1.0	55.1
psbM-petN_p5	TGCTACTGCACTGTTCATTT	TGGGCTGCTTTAATGGTAGTC	40.0	47.6	55.6	58.0	649-663 bp	1.0	54.3