Hotspot

rpoB-trnE-UUC

LSC · 71443-72189 · 747 bp

Back to Helichrysum

Pi 0.00937

MarkerSeek score 64.10

Primer available yes

Label rpoB-trnE-UUC

Feature Metadata

label_name: rpoB-trnE-UUC
feature_type: igs
region: LSC
length_bp: 747
variable_sites: 7
pi: 0.00937
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnE-UUC_p1	AAATCCAGGTATTGTGGCCA	GGGACTTGAACCCTCGTTTT	45.0	50.0	57.1	58.0	830-942 bp	1.0	73.1
rpoB-trnE-UUC_p2	AAATCCAGGTATTGTGGCCA	TGATAGGAAATCCCGCTCCT	45.0	50.0	57.1	57.9	878-990 bp	1.0	72.4
rpoB-trnE-UUC_p3	AAATCCAGGTATTGTGGCCA	TAACCACTGGACCATAGGGG	45.0	55.0	57.1	58.1	789-901 bp	1.0	72.4
rpoB-trnE-UUC_p4	AAATCCAGGTATTGTGGCCA	CCCTCGTTTTCTCCGTGAAA	45.0	50.0	57.1	58.1	820-932 bp	1.0	72.3
rpoB-trnE-UUC_p5	AAATCCAGGTATTGTGGCCA	TTGAACCCTCGTTTTCTCCG	45.0	50.0	57.1	58.1	825-937 bp	1.0	72.3