Hotspot

atpH-atpI

LSC · 58143-59265 · 1123 bp

Back to Helichrysum

Pi 0.00558

MarkerSeek score 66.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1123
variable_sites: 6
pi: 0.00558
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TCCCTGTCATGTTCCTTGGA	45.0	50.0	58.0	58.3	1224-1259 bp	1.0	81.0
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	TCCCTGTCATGTTCCTTGGA	50.0	50.0	57.9	58.3	1280-1315 bp	1.0	80.3
atpH-atpI_p3	AGCCAATCCAGCAGCAATAA	TCCCTGTCATGTTCCTTGGA	45.0	50.0	57.9	58.3	1240-1275 bp	1.0	80.1
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	TCCCTGTCATGTTCCTTGGA	45.0	50.0	58.2	58.3	1225-1260 bp	1.0	79.9
atpH-atpI_p5	GCAGTACCTTGACCAACTCC	TCCCTGTCATGTTCCTTGGA	55.0	50.0	58.2	58.3	1284-1319 bp	1.0	79.7