Hotspot

trnS-GGA-pafI

LSC · 38980-39904 · 925 bp

Back to Helichrysum

Pi 0.013

MarkerSeek score 62.20

Primer available yes

Label trnS-GGA-pafI

Feature Metadata

label_name: trnS-GGA-pafI
feature_type: igs
region: LSC
length_bp: 925
variable_sites: 12
pi: 0.013
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-pafI_p1	CCCTCGGTAAGCAAAAACCT	TCTCCTGAGGTTGTCGGAAT	50.0	50.0	58.1	58.1	1087-1090 bp	1.0	79.6
trnS-GGA-pafI_p2	TTCCAATGCTACGCCTTCAA	TCTCCTGAGGTTGTCGGAAT	45.0	50.0	57.8	58.1	1058-1061 bp	1.0	78.9
trnS-GGA-pafI_p3	GGATTCGAACCCTCGGTAAG	TCTCCTGAGGTTGTCGGAAT	55.0	50.0	57.8	58.1	1096-1099 bp	1.0	78.6
trnS-GGA-pafI_p4	AGTTCCAATGCTACGCCTTC	TCTCCTGAGGTTGTCGGAAT	50.0	50.0	58.3	58.1	1060-1063 bp	1.0	78.3
trnS-GGA-pafI_p5	CCCTCGGTAAGCAAAAACCT	TTCTCCTGAGGTTGTCGGAA	50.0	50.0	58.1	58.3	1088-1091 bp	1.0	77.8