Hotspot

trnV-UAC-ndhC

LSC · 32442-33692 · 1251 bp

Back to Helichrysum

Pi 0.00405

MarkerSeek score 62.40

Primer available yes

Label trnV-UAC-ndhC

Feature Metadata

label_name: trnV-UAC-ndhC
feature_type: igs
region: LSC
length_bp: 1251
variable_sites: 5
pi: 0.00405
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-ndhC_p1	GAGAAGGTCTACGGTTCGAG	TCCTTTGCTACTCGGAGCTA	55.0	50.0	57.2	57.9	1396-1443 bp	1.0	73.5
trnV-UAC-ndhC_p2	CGAGAAGGTCTACGGTTCGA	TCCTTTGCTACTCGGAGCTA	55.0	50.0	58.9	57.9	1397-1444 bp	1.0	72.8
trnV-UAC-ndhC_p3	GAGAAGGTCTACGGTTCGAG	ACTCGGAGCTAAAAATCCCG	55.0	50.0	57.2	57.7	1387-1434 bp	1.0	72.1
trnV-UAC-ndhC_p4	GAGAAGGTCTACGGTTCGAG	TTCTCCGGTCCTTTGCTACT	55.0	50.0	57.2	58.4	1404-1451 bp	1.0	71.7
trnV-UAC-ndhC_p5	CGAGAAGGTCTACGGTTCGA	ACTCGGAGCTAAAAATCCCG	55.0	50.0	58.9	57.7	1388-1435 bp	1.0	71.4