Hotspot

atpE-trnM-CAU

LSC · 31331-31544 · 214 bp

Back to Helichrysum

Pi 0.014

MarkerSeek score 52.90

Primer available yes

Label atpE-trnM-CAU

Feature Metadata

label_name: atpE-trnM-CAU
feature_type: igs
region: LSC
length_bp: 214
variable_sites: 3
pi: 0.014
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpE-trnM-CAU_p1	CAAATCTAGCTCTCCGACGG	GCGGGAGTCATTGGTTCAAA	55.0	50.0	57.9	58.8	307 bp	1.0	73.7
atpE-trnM-CAU_p2	GGCAAATCTAGCTCTCCGAC	GCGGGAGTCATTGGTTCAAA	55.0	50.0	58.1	58.8	309 bp	1.0	73.6
atpE-trnM-CAU_p3	GAGGCAAATCTAGCTCTCCG	GCGGGAGTCATTGGTTCAAA	55.0	50.0	57.9	58.8	311 bp	1.0	73.5
atpE-trnM-CAU_p4	AGGCAAATCTAGCTCTCCGA	GCGGGAGTCATTGGTTCAAA	50.0	50.0	58.2	58.8	310 bp	1.0	73.0
atpE-trnM-CAU_p5	CTAGGACACGAGTAGAGGCT	GCGGGAGTCATTGGTTCAAA	55.0	50.0	57.7	58.8	286 bp	1.0	72.1