Hotspot

rbcL-atpB

LSC · 28705-29444 · 740 bp

Back to Helichrysum

Pi 0.00541

MarkerSeek score 61.90

Primer available yes

Label rbcL-atpB

Feature Metadata

label_name: rbcL-atpB
feature_type: igs
region: LSC
length_bp: 740
variable_sites: 4
pi: 0.00541
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-atpB_p1	CACCTGCTTTGAATCCAACAC	ATACGCCCCAGGTCATTTTC	47.6	50.0	58.0	57.9	839-848 bp	1.0	72.7
rbcL-atpB_p2	CACCTGCTTTGAATCCAACAC	GATACGCCCCAGGTCATTTT	47.6	50.0	58.0	57.9	840-849 bp	1.0	72.7
rbcL-atpB_p3	ACCTGCTTTGAATCCAACACT	ATACGCCCCAGGTCATTTTC	42.9	50.0	58.1	57.9	838-847 bp	1.0	72.4
rbcL-atpB_p4	ACCTGCTTTGAATCCAACACT	GATACGCCCCAGGTCATTTT	42.9	50.0	58.1	57.9	839-848 bp	1.0	72.4
rbcL-atpB_p5	TGCTTTGAATCCAACACTTGC	ATACGCCCCAGGTCATTTTC	42.9	50.0	57.9	57.9	835-844 bp	1.0	72.0