Hotspot

psaI-accD

LSC · 24508-25221 · 714 bp

Back to Helichrysum

Pi 0.0129

MarkerSeek score 68.00

Primer available yes

Label psaI-accD

Feature Metadata

label_name: psaI-accD
feature_type: igs
region: LSC
length_bp: 714
variable_sites: 9
pi: 0.0129
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-accD_p1	TTGCGATTGCCGGAAATACT	TCCAGAAGGGCTTATTCGATC	45.0	47.6	58.3	57.2	871-885 bp	1.0	66.0
psaI-accD_p2	TTGCGATTGCCGGAAATACT	CCAGAAGGGCTTATTCGATCT	45.0	47.6	58.3	57.2	870-884 bp	1.0	66.0
psaI-accD_p3	TTGCGATTGCCGGAAATACT	TTCCAGAAGGGCTTATTCGA	45.0	45.0	58.3	56.2	872-886 bp	1.0	66.0
psaI-accD_p4	AGGCCTACTAAAGGGACCAA	TCCAGAAGGGCTTATTCGATC	50.0	47.6	57.6	57.2	851-865 bp	1.0	65.3
psaI-accD_p5	AGGCCTACTAAAGGGACCAA	CCAGAAGGGCTTATTCGATCT	50.0	47.6	57.6	57.2	850-864 bp	1.0	65.2