Hotspot

psbJ-petA

LSC · 20351-21151 · 801 bp

Back to Helichrysum

Pi 0.0175

MarkerSeek score 60.10

Primer available yes

Label psbJ-petA

Feature Metadata

label_name: psbJ-petA
feature_type: igs
region: LSC
length_bp: 801
variable_sites: 14
pi: 0.0175
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-petA_p1	ATTCCGGATTGGGTTCATCC	CGAGAAGGTTCAATTGTCCG	50.0	50.0	57.6	56.8	861-869 bp	1.0	69.1
psbJ-petA_p2	ATTCCGGATTGGGTTCATCC	CGAGAAGGTTCAATTGTCCGA	50.0	47.6	57.6	58.3	861-869 bp	1.0	67.8
psbJ-petA_p3	ATTCCGGATTGGGTTCATCC	TCGAGAAGGTTCAATTGTCCG	50.0	47.6	57.6	58.3	862-870 bp	1.0	67.8
psbJ-petA_p4	TGTGATCGGTTTAGTAGGCG	CGAGAAGGTTCAATTGTCCG	50.0	50.0	57.4	56.8	899-907 bp	1.0	67.4
psbJ-petA_p5	GTGATCGGTTTAGTAGGCGT	CGAGAAGGTTCAATTGTCCG	50.0	50.0	57.4	56.8	898-906 bp	1.0	67.4