Hotspot

rpl33-psaJ

LSC · 16549-16994 · 446 bp

Back to Helichrysum

Pi 0.00686

MarkerSeek score 61.40

Primer available yes

Label rpl33-psaJ

Feature Metadata

label_name: rpl33-psaJ
feature_type: igs
region: LSC
length_bp: 446
variable_sites: 3
pi: 0.00686
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-psaJ_p1	AGAACCGGTATTCGCACATC	TGTGGCACCGGTACTAAGTA	50.0	50.0	58.1	58.1	581-590 bp	1.0	80.5
rpl33-psaJ_p2	GAACCGGTATTCGCACATCT	TGTGGCACCGGTACTAAGTA	50.0	50.0	58.1	58.1	580-589 bp	1.0	80.5
rpl33-psaJ_p3	TTTCGAACACAAGCGGTACA	TGTGGCACCGGTACTAAGTA	45.0	50.0	58.1	58.1	608-617 bp	1.0	80.4
rpl33-psaJ_p4	TCGCACATCTTTACCCTTGG	TGTGGCACCGGTACTAAGTA	50.0	50.0	57.9	58.1	570-579 bp	1.0	80.2
rpl33-psaJ_p5	TCGAACACAAGCGGTACATT	TGTGGCACCGGTACTAAGTA	45.0	50.0	57.8	58.1	606-615 bp	1.0	79.7