Hotspot

infA-rpl36

LSC · 4374-4495 · 122 bp

Back to Helichrysum

Pi 0.0164

MarkerSeek score 61.90

Primer available yes

Label infA-rpl36

Feature Metadata

label_name: infA-rpl36
feature_type: igs
region: LSC
length_bp: 122
variable_sites: 2
pi: 0.0164
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rpl36_p1	ACTCCGCAACAAAGATTCGA	TTTTACGAACGGAAGCTCTT	45.0	40.0	57.8	55.3	178 bp	1.0	58.3
infA-rpl36_p2	TCGACTCCGCAACAAAGATT	TTTTACGAACGGAAGCTCTT	45.0	40.0	57.8	55.3	181 bp	1.0	58.3
infA-rpl36_p3	ACTCCGCAACAAAGATTCGA	ACAAATTTTACGAACGGAAGCT	45.0	36.4	57.8	57.2	183 bp	1.0	57.0
infA-rpl36_p4	TCGACTCCGCAACAAAGATT	ACAAATTTTACGAACGGAAGCT	45.0	36.4	57.8	57.2	186 bp	1.0	57.0
infA-rpl36_p5	ACTCCGCAACAAAGATTCGA	CACAAATTTTACGAACGGAAGC	45.0	40.9	57.8	57.1	184 bp	1.0	56.7