Hotspot

rps16

LSC · 5221-6355 · 1135 bp

Back to Helianthus

Pi 0.00223

MarkerSeek score 61.20

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1135
variable_sites: 23
pi: 0.00223
unique_haplotype_count: 32
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	ACGAATAGAGCAAGAAAAGAAGG	TGACTGTGTCTCTGTACCGA	39.1	50.0	56.9	57.7	1286-1336 bp	1.0	49.1
rps16_p2	ACGAATAGAGCAAGAAAAGAAGG	TGTGTCTCTGTACCGACTGA	39.1	50.0	56.9	57.7	1282-1332 bp	1.0	49.1
rps16_p3	ACGAATAGAGCAAGAAAAGAAGG	ACTGTGTCTCTGTACCGACT	39.1	50.0	56.9	57.7	1284-1334 bp	1.0	49.0
rps16_p4	ACGAATAGAGCAAGAAAAGAAGG	TCTCTGTACCGACTGAACCA	39.1	50.0	56.9	57.7	1278-1328 bp	1.0	49.0
rps16_p5	ACGAATAGAGCAAGAAAAGAAGGA	TGTGTCTCTGTACCGACTGA	37.5	50.0	58.2	57.7	1282-1332 bp	1.0	48.4