Hotspot

clpP1

LSC · 69402-71397 · 1996 bp

Back to Helianthus

Pi 0.00151

MarkerSeek score 51.50

Primer available yes

Label clpP1

Feature Metadata

label_name: clpP1
feature_type: gene
region: LSC
length_bp: 1996
variable_sites: 39
pi: 0.00151
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1_p1	AAACTCGGCAGGGTGAATTT	GGCTTTGTTACGTTTCCACA	45.0	45.0	58.0	56.9	1919-2115 bp	0.972973	75.3
clpP1_p2	AAACTCGGCAGGGTGAATTT	AGGCTTTGTTACGTTTCCACA	45.0	42.9	58.0	58.4	1920-2116 bp	0.972973	73.6
clpP1_p3	AAACTCGGCAGGGTGAATTT	AGGCTTTGTTACGTTTCCAC	45.0	45.0	58.0	56.6	1920-2116 bp	0.972973	72.9
clpP1_p4	AACTCGGCAGGGTGAATTTC	GGCTTTGTTACGTTTCCACA	50.0	45.0	58.5	56.9	1918-2114 bp	0.972973	71.6
clpP1_p5	CGGCAGGGTGAATTTCAGAA	GGCTTTGTTACGTTTCCACA	50.0	45.0	58.5	56.9	1914-2110 bp	0.972973	71.6