Hotspot

rrn5-trnR-ACG

IRb · 106408-106683 · 276 bp

Back to Helianthus

Pi 0.008

MarkerSeek score 59.90

Primer available yes

Label rrn5-trnR-ACG

Feature Metadata

label_name: rrn5-trnR-ACG
feature_type: igs
region: IRb
length_bp: 276
variable_sites: 17
pi: 0.008
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn5-trnR-ACG_p1	GGTGACGATACTGTAGGGGA	CCACGTGCTCTAATCCTCTG	55.0	55.0	57.9	57.8	336-395 bp	1.0	85.6
rrn5-trnR-ACG_p2	AATCCATCCCGAACTTGGTG	CCACGTGCTCTAATCCTCTG	50.0	55.0	57.9	57.8	371-430 bp	1.0	84.7
rrn5-trnR-ACG_p3	ACCAATCCATCCCGAACTTG	CCACGTGCTCTAATCCTCTG	50.0	55.0	57.9	57.8	374-433 bp	1.0	84.7
rrn5-trnR-ACG_p4	CAATCCATCCCGAACTTGGT	CCACGTGCTCTAATCCTCTG	50.0	55.0	57.9	57.8	372-431 bp	1.0	84.7
rrn5-trnR-ACG_p5	CACCAATCCATCCCGAACTT	CCACGTGCTCTAATCCTCTG	50.0	55.0	57.9	57.8	375-434 bp	1.0	84.7