Hotspot

rpl14-rpl16

LSC · 80539-80644 · 106 bp

Back to Helianthus

Pi 0.0112

MarkerSeek score 59.20

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 106
variable_sites: 17
pi: 0.0112
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	ACACATCAATTCTCGAGCCC	AATAGCAGCGTCCAAAATGC	50.0	45.0	58.0	57.4	221-254 bp	1.0	84.7
rpl14-rpl16_p2	ACACATCAATTCTCGAGCCC	GCGTCCAAAATGCCTATACG	50.0	50.0	58.0	57.3	214-247 bp	1.0	84.2
rpl14-rpl16_p3	TCGACGATTGCTAGCTCCTA	AATAGCAGCGTCCAAAATGC	50.0	45.0	58.0	57.4	251-284 bp	1.0	83.6
rpl14-rpl16_p4	TCGACGATTGCTAGCTCCTA	GCGTCCAAAATGCCTATACG	50.0	50.0	58.0	57.3	244-277 bp	1.0	83.0
rpl14-rpl16_p5	CCCGCTGTTATCTGCTACAT	AATAGCAGCGTCCAAAATGC	50.0	45.0	57.5	57.4	203-236 bp	1.0	81.5