Hotspot

trnP-UGG-psaJ

LSC · 66265-66583 · 319 bp

Back to Helianthus

Pi 0.00311

MarkerSeek score 33.30

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 319
variable_sites: 7
pi: 0.00311
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	GGTGCCACGGAAAGATATGT	50.0	50.0	58.1	58.0	409-416 bp	1.0	80.3
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	GGTGCCACGGAAAGATATGT	50.0	50.0	58.1	58.0	408-415 bp	1.0	80.3
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	GGTGCCACGGAAAGATATGT	50.0	50.0	58.1	58.0	420-427 bp	1.0	80.2
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	GGTGCCACGGAAAGATATGT	50.0	50.0	58.1	58.0	419-426 bp	1.0	80.2
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	GGTGCCACGGAAAGATATGT	50.0	50.0	58.1	58.0	422-429 bp	1.0	80.1