Hotspot

atpB-rbcL

LSC · 53925-54677 · 753 bp

Back to Helianthus

Pi 0.0033

MarkerSeek score 58.90

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 753
variable_sites: 17
pi: 0.0033
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GGCAACCCCAGAACTAGAAG	CACCAGCTTTGAATCCAACAC	55.0	47.6	57.9	58.0	746-949 bp	1.0	76.5
atpB-rbcL_p2	GATACGCCCCAGGTTCTTTT	CACCAGCTTTGAATCCAACAC	50.0	47.6	57.9	58.0	776-979 bp	1.0	76.5
atpB-rbcL_p3	GGCAACCCCAGAACTAGAAG	ACCAGCTTTGAATCCAACACT	55.0	42.9	57.9	58.1	745-948 bp	1.0	76.3
atpB-rbcL_p4	GGCGGAAAGGCTACATCTAG	CACCAGCTTTGAATCCAACAC	55.0	47.6	57.8	58.0	817-1020 bp	1.0	76.2
atpB-rbcL_p5	GATACGCCCCAGGTTCTTTT	ACCAGCTTTGAATCCAACACT	50.0	42.9	57.9	58.1	775-978 bp	1.0	76.2