Hotspot

ndhC-trnV-UAC

LSC · 49970-50911 · 942 bp

Back to Helianthus

Pi 0.0029
MarkerSeek score 59.20
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
942
variable_sites
28
pi
0.0029
unique_haplotype_count
23
primer_available
yes
alignment_reliability
0.97
markerseek_score
59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TTCTCCGGTCCTTTGCTACT AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.4 57.8 1055-1107 bp 1.0 81.6
ndhC-trnV-UAC_p2 CTTTGCTACTCGGGGCTAAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.6 57.8 1045-1097 bp 1.0 81.4
ndhC-trnV-UAC_p3 TTTCTCCGGTCCTTTGCTAC AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.5 57.8 1056-1108 bp 1.0 80.8
ndhC-trnV-UAC_p4 TTCTCCGGTCCTTTGCTACT GAAGGTCTACGGTTCGAGTC 50.0 55.0 58.4 57.5 1054-1106 bp 1.0 78.9
ndhC-trnV-UAC_p5 CTTTGCTACTCGGGGCTAAA GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.6 57.5 1044-1096 bp 1.0 78.6