Hotspot

psaA-pafI

LSC · 41601-42362 · 762 bp

Back to Helianthus

Pi 0.0033

MarkerSeek score 59.70

Primer available yes

Label psaA-pafI

Feature Metadata

label_name: psaA-pafI
feature_type: igs
region: LSC
length_bp: 762
variable_sites: 17
pi: 0.0033
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-pafI_p1	GAGAAATGACCCGGTCTAGC	AGCCGCTGAGTATTGGAAAC	55.0	50.0	57.8	58.3	908-954 bp	1.0	81.6
psaA-pafI_p2	GAGAAATGACCCGGTCTAGC	CAAGCCGCTGAGTATTGGAA	55.0	50.0	57.8	58.3	910-956 bp	1.0	81.6
psaA-pafI_p3	GAGAAATGACCCGGTCTAGC	AAGCCGCTGAGTATTGGAAA	55.0	45.0	57.8	57.5	909-955 bp	1.0	79.7
psaA-pafI_p4	TTTTACTTCTGGTTCCGGCG	AGCCGCTGAGTATTGGAAAC	50.0	50.0	58.5	58.3	843-889 bp	1.0	79.6
psaA-pafI_p5	TTTTACTTCTGGTTCCGGCG	CAAGCCGCTGAGTATTGGAA	50.0	50.0	58.5	58.3	845-891 bp	1.0	79.6