Hotspot

rpoC2

LSC · 17504-21673 · 4170 bp

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Pi 0.00024
MarkerSeek score 53.90
Primer available yes
Label rpoC2

Feature Metadata

label_name
rpoC2
feature_type
gene
region
LSC
length_bp
4170
variable_sites
1
pi
0.00024
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC2_p1 TGTGTCAGAAATTCCCCGAA GGCATCTTGATACCACCAGG 45.0 55.0 57.1 58.0 270 bp 1.0 72.6
rpoC2_p2 TTGTGTCAGAAATTCCCCGA GGCATCTTGATACCACCAGG 45.0 55.0 57.1 58.0 271 bp 1.0 72.6
rpoC2_p3 TGTGTCAGAAATTCCCCGAA CGACACAGTGGCATCTTGAT 45.0 50.0 57.1 58.3 279 bp 1.0 70.6
rpoC2_p4 TTGTGTCAGAAATTCCCCGA CGACACAGTGGCATCTTGAT 45.0 50.0 57.1 58.3 280 bp 1.0 70.6
rpoC2_p5 TTGTGTCAGAAATTCCCCGAA GGCATCTTGATACCACCAGG 42.9 55.0 57.8 58.0 271 bp 1.0 70.3