Hotspot

psbA

LSC · 384-1445 · 1062 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label psbA

Feature Metadata

label_name
psbA
feature_type
gene
region
LSC
length_bp
1062
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA_p1 TCAAGAGTGTTGATTATTGCTCC AGAATTCGTGTGCTTGGGAG 39.1 50.0 56.9 58.2 1186 bp 1.0 45.4
psbA_p2 TCAAGAGTGTTGATTATTGCTCC AGAGAATTCGTGTGCTTGGG 39.1 50.0 56.9 58.2 1188 bp 1.0 45.4
psbA_p3 TCAAGAGTGTTGATTATTGCTCC GAGAATTCGTGTGCTTGGGA 39.1 50.0 56.9 58.2 1187 bp 1.0 45.4
psbA_p4 CAAGAGTGTTGATTATTGCTCCT AGAATTCGTGTGCTTGGGAG 39.1 50.0 56.9 58.2 1185 bp 1.0 45.3
psbA_p5 CAAGAGTGTTGATTATTGCTCCT AGAGAATTCGTGTGCTTGGG 39.1 50.0 56.9 58.2 1187 bp 1.0 45.3