Hotspot

accD-psaI

LSC · 62304-62956 · 653 bp

Back to Heimia

Pi 0.00153
MarkerSeek score 59.60
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
653
variable_sites
1
pi
0.00153
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 ACGCCTTTTTCCCCTTGAAT CCATTGCAATTGCCGGAAAT 45.0 45.0 58.0 58.0 761 bp 1.0 79.8
accD-psaI_p2 GAAGCTTCACGCCTTTTTCC CCATTGCAATTGCCGGAAAT 50.0 45.0 57.9 58.0 769 bp 1.0 79.5
accD-psaI_p3 ACGCCTTTTTCCCCTTGAAT AAGAAGCCATTGCAATTGCC 45.0 45.0 58.0 57.9 767 bp 1.0 79.2
accD-psaI_p4 GAAGCTTCACGCCTTTTTCC AAGAAGCCATTGCAATTGCC 50.0 45.0 57.9 57.9 775 bp 1.0 78.9
accD-psaI_p5 ACGCCTTTTTCCCCTTGAAT GCCGGAAATACTAGGCCTAC 45.0 55.0 58.0 57.5 750 bp 1.0 76.4