Hotspot

matK-trnK

LSC · 3504-4241 · 738 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label matK-trnK

Feature Metadata

label_name
matK-trnK
feature_type
igs
region
LSC
length_bp
738
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK_p1 AGGGGGTATAGGAAGTCGTG AACGGTAGAGTACTCGGCTT 55.0 50.0 57.9 58.2 827 bp 1.0 78.0
matK-trnK_p2 AGGGGGTATAGGAAGTCGTG ACGGTAGAGTACTCGGCTTT 55.0 50.0 57.9 58.2 826 bp 1.0 78.0
matK-trnK_p3 GAAGTCGTGGTGTCGAGATC AACGGTAGAGTACTCGGCTT 55.0 50.0 58.1 58.2 816 bp 1.0 77.8
matK-trnK_p4 GAAGTCGTGGTGTCGAGATC ACGGTAGAGTACTCGGCTTT 55.0 50.0 58.1 58.2 815 bp 1.0 77.8
matK-trnK_p5 ATAGGAAGTCGTGGTGTCGA ACGGTAGAGTACTCGGCTTT 50.0 50.0 58.2 58.2 819 bp 1.0 77.3