Hotspot

clpP

Genome · 66247-66876 · 630 bp

Back to Hedysarum

Pi 0.105

MarkerSeek score 80.70

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: Genome
length_bp: 630
variable_sites: 252
pi: 0.105
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CCCTCTTAGTCCGTTCTTGC	AAAAGCACAAGCCCGAAAAC	55.0	45.0	58.0	58.1	1680-2532 bp	1.0	91.4
clpP_p2	CCCTCTTAGTCCGTTCTTGC	ACGAACATACCCTGTCTCCA	55.0	50.0	58.0	58.1	1540-2392 bp	1.0	91.4
clpP_p3	CCCTCTTAGTCCGTTCTTGC	CAAAAAGCACAAGCCCGAAA	55.0	45.0	58.0	58.1	1682-1973 bp	0.92	88.2
clpP_p4	AGAAACACACGACAGCCAAT	AAAAGCACAAGCCCGAAAAC	45.0	45.0	58.0	58.1	1263-2108 bp	0.56	73.6
clpP_p5	AGAAACACACGACAGCCAAT	CAAAAAGCACAAGCCCGAAA	45.0	45.0	58.0	58.1	1265-1369 bp	0.48	70.4