Hotspot

accD

Genome · 54654-55823 · 1170 bp

Back to Hedysarum

Pi 0.0393

MarkerSeek score 74.20

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: Genome
length_bp: 1170
variable_sites: 295
pi: 0.0393
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	CCTTCCGTCCCAGATCCTAT	AAGAAGCCATTGCAATTGCC	55.0	45.0	58.0	57.9	2050-2517 bp	0.92	87.0
accD_p2	CCTTCCGTCCCAGATCCTAT	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	2044-2511 bp	0.88	85.8
accD_p3	GAGGTTCGAATCCTTCCGTC	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.1	58.0	2055-2522 bp	0.88	85.3
accD_p4	CCTTCCGTCCCAGATCCTAT	ACTGGATGAATTTCGCCCTC	55.0	50.0	58.0	58.0	1607-1876 bp	0.8	80.0
accD_p5	GAGGTTCGAATCCTTCCGTC	ACTGGATGAATTTCGCCCTC	55.0	50.0	58.1	58.0	1618-1887 bp	0.8	79.5