Hotspot

trnN-GUU-ycf1

Genome · 102856-103513 · 658 bp

Back to Hedysarum

Pi 0.0916

MarkerSeek score 75.80

Primer available yes

Label trnN-GUU-ycf1

Feature Metadata

label_name: trnN-GUU-ycf1
feature_type: igs
region: Genome
length_bp: 658
variable_sites: 181
pi: 0.0916
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.73
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ycf1_p1	GGCGGACCCTAAGCTATAGA	CTTTTTCCATAACCCGGGCT	55.0	50.0	58.1	58.2	1046-1486 bp	0.88	85.3
trnN-GUU-ycf1_p2	GGCGGACCCTAAGCTATAGA	AAACCCAGTTGTTGCTGCTA	55.0	45.0	58.1	57.9	1081-1462 bp	0.84	84.2
trnN-GUU-ycf1_p3	GGCGGACCCTAAGCTATAGA	TAAACCCAGTTGTTGCTGCT	55.0	45.0	58.1	57.9	1082-1463 bp	0.84	84.2
trnN-GUU-ycf1_p4	GGCGGACCCTAAGCTATAGA	AAGAAGGCCCTACGGAGAAT	55.0	50.0	58.1	58.1	1010-1391 bp	0.84	84.0
trnN-GUU-ycf1_p5	GGCGGACCCTAAGCTATAGA	ACGGTAGAGTTAGGGCAGTT	55.0	50.0	58.1	58.1	1188-1618 bp	0.8	82.8