Hotspot

trnI-CAU-ycf2

Genome · 81844-83930 · 2087 bp

Back to Hedysarum

Pi 0.0531

MarkerSeek score 83.70

Primer available yes

Label trnI-CAU-ycf2

Feature Metadata

label_name: trnI-CAU-ycf2
feature_type: igs
region: Genome
length_bp: 2087
variable_sites: 445
pi: 0.0531
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-ycf2_p1	ATTGAACCTACGACTTCGCC	TGGTGATATTCTTCCTCCATTGA	50.0	39.1	58.0	57.0	1897-2241 bp	0.92	57.0
trnI-CAU-ycf2_p2	GAACCTACGACTTCGCCAAT	TGGTGATATTCTTCCTCCATTGA	50.0	39.1	58.0	57.0	1894-2238 bp	0.92	57.0
trnI-CAU-ycf2_p3	GTTGGGCGCTTTAACCATTC	TGGTGATATTCTTCCTCCATTGA	50.0	39.1	58.0	57.0	1869-2213 bp	0.92	56.9
trnI-CAU-ycf2_p4	AGTTGGGCGCTTTAACCATT	TGGTGATATTCTTCCTCCATTGA	45.0	39.1	58.4	57.0	1870-2214 bp	0.92	54.0
trnI-CAU-ycf2_p5	TTGGGCGCTTTAACCATTCA	TGGTGATATTCTTCCTCCATTGA	45.0	39.1	58.4	57.0	1868-2212 bp	0.92	54.0