Hotspot

trnP-UGG-psaJ

Genome · 62951-63358 · 408 bp

Back to Hedysarum

Pi 0.034

MarkerSeek score 79.30

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: Genome
length_bp: 408
variable_sites: 71
pi: 0.034
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	AAAACAAACGCGCTACCAAG	ACTGGTGCCACAGAGAGATA	45.0	50.0	57.9	57.8	480-665 bp	1.0	89.2
trnP-UGG-psaJ_p2	CAAAACAAACGCGCTACCAA	ACTGGTGCCACAGAGAGATA	45.0	50.0	58.2	57.8	481-666 bp	1.0	88.7
trnP-UGG-psaJ_p3	AAAACAAACGCGCTACCAAG	CCGCTAAAATCCCGAACCAT	45.0	50.0	57.9	58.3	512-697 bp	1.0	88.5
trnP-UGG-psaJ_p4	CAAAACAAACGCGCTACCAA	CCGCTAAAATCCCGAACCAT	45.0	50.0	58.2	58.3	513-698 bp	1.0	88.0
trnP-UGG-psaJ_p5	TTGTACCCAAAACAAACGCG	ACTGGTGCCACAGAGAGATA	45.0	50.0	57.8	57.8	488-673 bp	0.88	83.8